RESEARCH
細胞生物学
キーワード：#細胞運動 #シグナル伝達 #細胞骨格 #ライブイメージング
細胞機能を分子レベルで解明する
私たちは、生物の高次機能を解明するため、動物細胞の運動のメカニズムを細胞、分子レベルで研究しています。細胞の運動は動物の発生や免疫反応などの生物の基本的な高次機能に関わるとともに、精神遅滞やがん細胞の浸潤?転移などの病気にも深く関与しています。私たちは細胞生物学の研究によって生物の高次機能と病気の原因解明に貢献したいと考えています。
業績
Improvement of the green-red F?rster resonance energy transfer-based Ca2+ indicator by using the green fluorescent protein, Gamillus, with a trans chromophore as the donor
Matsuda, T., Sakai, S., Okazaki, K. I., & Nagai, T. ACS Sens. 2024 Apr 26;9(4):1743-1748. doi: 10.1021/acssensors.3c02398
Near-infrared PAINT localization microscopy via chromophore replenishment of phytochrome-derived fluorescent tag
Lu, K., Wazawa, T., Matsuda, T., Shcherbakova, D. M., Verkhusha, V. V., & Nagai, T., Commun Biol. 2024;7(1):473. 2024 Apr 18. doi:10.1038/s42003-024-06169-7
FilGAP controls cell-extracellular matrix adhesion and process formation of kidney podocytes.
Saito, K., Yokawa, S., Kurihara, H., Yaoita, E., Mizuta, S., Tada, K., Oda, M., Hatakeyama, H., & Ohta, Y. FASEB J. 2024 Mar 15;38(5):e23504. doi: 10.1096/fj.202301691RR.
RSK/GSK3 mediated phosphorylation of FilGAP regulates chemotactic cancer cell invasion.
Tsutsumi, K.& Ohta, Y. PNAS Nexus, doi.org/10.1093/pnasnexus/pgae071
FilGAP regulates tumor growth in Glioma through the regulation of mTORC1 and mTORC2.
Tsutsumi, K., Nohara, A., Tanaka, T., Murano, M., Miyagaki, Y., & Ohta, Y. Sci Rep. 2023 Dec 8;13(1):20956. doi: 10.1038/s41598-023-47892-1.
FilGAP, a GAP for Rac1, down-regulates invadopodia formation in breast cancer cells.
Saito, K., Ozawa, S., Chiba, Y., Takahashi, R., Ogomori, R., Mukai, K., Taguchi, T., Hatakeyama, H., & Ohta, Y. Cell Struct. Funct. https://doi.org/10.1247/csf.23032
Endosomal Localization of RacGAP Protein ARHGAP22 Regulates its GAP Activity in Human Melanoma Cells.
Mori, M., Saito, K., Sekine, A., Hasebe, R., & Ohta, Y. Anticancer Res. 2022; 42: 5763-5771.
FilGAP, a GAP protein for Rac, regulates front-rear polarity and tumor cell migration through the ECM.
Saito K., Mori M., Kambara N., & Ohta Y. The FASEB journal 2021; 35: e21508. doi: 10.1096/fj.202002155R.
Involvement of Nlrp9a/b/c in mouse preimplantation development.
Kanzaki S., Tamura S., Ito T., Wakabayashi M., Saito K., Kato S., Ohta Y., Sekita Y., & Kimura T. Reproduction 2020; 160: 181-191
AGAP1 regulates subcellular localization of FilGAP and control cancer cell invasion.
Tsutsumi, K., Nakamura Y., Kitagawa Y., Suzuki Y., Shibagaki Y., Hattori S., & Ohta Y. Biochem Biophys Res Commun. 2020; 522: 676-683
FilGAP regulates distinct stages of epithelial tubulogenesis.
Zuinen, T., Tsutsumi, K, & Ohta Y. Biochem Biophys Res Commun. 2019; 514: 742-749
Role of ARHGAP24 in ADP ribosylation factor 6 (ARF6)-dependent pseudopod formation in human breast carcinoma cells.
Uehara, S., Saito, K., Asami, H., & Ohta Y. Anticancer Res. 2017; 37: 4837-4844.
The role of FilGAP, a Rac-specific Rho GTPase-activating protein, in tumor progression and behavior of astrocytomas.
Hara, A., Hashimura, M., Tsutsumi, K, Akiya, M., Inukai, M., Ohta, Y., & Saegusa, M. Cancer Med. 2016; 5: 3412-3425
The RacGAP protein FilGAP is a negative regulator of chemokine-promoted lymphocyte migration.
Iida, T., Saito, K., Katagiri, K., Kinashi, T., & Ohta Y. FEBS Lett. 2016 Apr 30. doi: 10.1002/1873-3468.12189.
Src Family Tyrosine Kinase Signaling Regulates FilGAP through Association with RBM10.
Yamada, H., Tsutsumi, K., Nakazawa, Y., Shibagaki, Y., Hattori, S., & Ohta, Y. PLOS ONE 2016; 10.1371/journal.pone.0146593
Afadin is localized at cell-cell contact sites in mesangial cells and regulates migratory polarity.
Tsurumi, H., Kurihara, H., Miura, K., Tanego, A., Ohta, Y., Igarashi, T., Oka, A., Horita, S., Hattori, M., Harita, Y. Lab Invest. 2016; 96: 49-59
Phosphorylation of Serine 402 Regulates RacGAP Activity of FilGAP.
Morishita, Y., Tsutsumi, K., & Ohta, Y. J. Biol. Chem. 2015; 290:26328-26338
FilGAP, a Rho/ROCK-regulated GAP for Rac controls adherens junctions in MDCK cells.
Nakahara, S., Tsutsumi, K., Zuinen, Y., & Ohta, Y. J. Cell Sci. 2015; 128: 2047-2056
Afadin regulates RhoA/Rho-associated protein kinase signaling to control formation of actin stress fibers in kidney podocytes.
Saito, K., Shiino, T., Kurihara, H., Harita, Y., Hattori, S., & Ohta, Y. Cytoskeleton (Hoboken). 2015; 72: 146-156
ARHGAP22 localizes at endosomes and regulates actin cytoskeleton.
Mori, M., Saito, K., & Ohta, Y. PLoS ONE 2014; 9 (6): e100271
Arf6 acts through FilGAP to down-regulate Rac and regulates plasma membrane blebbing.
Kawaguchi, K., Saito, K., Asami, H., & Ohta, Y. J. Biol. Chem. 2014; 289: 9675-9682
FilGAP, a Rho/Rho-associated protein kinase-regulated GTPase-activating protein for Rac, controls tumor cell migration.
Saito, K., Ozawa, Y., Hibino, Y., & Ohta, Y. Mol. Biol. Cell 2012; 23: 4739-4750
