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和漢薬物学研究室

  • 研究の概要
  • メンバー
  • 研究業績

研究の概要

漢方薬などの和漢薬は小児、高齢者や慢性疾患罹患患者などの免疫弱者での難治性市中感染症および肺炎などの付随疾患とともにアレルギー性疾患などの西洋医学での治療のみでは著効が得られていない種々の疾患に対しその有用性が期待されている。本研究室では、このような背景と臨床知見をもとに、漢方薬などの和漢薬の感染症および重要疾病の予防?治療への高度利用を目指し、特に粘膜免疫機構制御の観点から薬効発現機序の解析や新しい概念の創薬への利用に関する以下の研究を行っている。

漢方薬などの和漢薬の感染症ならびに難治性疾患の予防および治療効果に関する薬効発現機序の解析と応用

  1. 漢方薬の薬理作用，作用機序および作用成分の総合的な解析に関する研究
    (1) 粘膜免疫機構制御の観点からの免疫低下および慢性炎症等の改善を指向した漢方薬の薬効解析
    (2) インフルエンザウイルス感染など感染症に対する漢方薬の薬効の総合的解析
    (3) 気分障害に対する漢方薬の薬効の総合的解析
  2. 和漢薬の薬効成分としての高分子糖鎖化合物（多糖）の構造と腸管免疫系を介した活性発現メカニズムの解析
  3. 和漢薬等の薬効成分情報を基にした感染防御応答や粘膜免疫機構応答の制御のための医薬および有用シーズの開発
マウスへの抗がん剤「メソトレキセート」投与による腸上皮障害に対す る補中益気湯の改善効果

マウスへの抗がん剤「メソトレキセート」投与による腸上皮障害に対す る補中益気湯の改善効果
Improving effect of hochuekkito on tissue damage in jejunum of methotrexate- treated mouse

インフルエンザウイルスを感染させたマウスの気道（鼻腔及び肺）に おける麻黄湯のウイルス増殖抑制効果

インフルエンザウイルスを感染させたマウスの気道（鼻腔及び肺）に おける麻黄湯のウイルス増殖抑制効果
Anti-influenza virus activity of maoto in the respiratory tract (nasal and bronchoalveolar cavities) of virus-infected mice

教職員?大学院学生

教員

清原 寛章
清原 寛章
教授
永井 隆之
永井 隆之
准教授

大学院生

  • 岸本 大志
    感染制御科学府　博士前期（修士）課程
  • 笹生 香耶子
    感染制御科学府　博士前期（修士）課程
  • 歌川 詩織
    感染制御科学府　博士前期（修士）課程
  • 田中 遥友
    感染制御科学府　博士前期（修士）課程
  • 殿岡 実樹
    感染制御科学府　博士前期（修士）課程
  • 前田 拓也
    感染制御科学府　博士前期（修士）課程
  • 山元 麻里奈
    感染制御科学府　博士前期（修士）課程

研究業績

平成２８（２０１６）年度

  1. Shirahata T., Nagai T., Hirata N., Yokoyama M., Katsumi T., Konishi N., Nishino T., Makino K., Yamada H., Kaji E., Kiyohara H., Kobayashi Y.: Syntheses and mucosal adjuvant activity of simplified oleanolic acid saponins possessing cinnamoyl ester. Bioorg. Med. Chem. 25 (6), 1747-1755 (2017).
  2. Chiba T., Asami Y., Suga T., Watanabe Y., Nagai T., Momose F., Nonaka K., Iwatsuki M., Yamada H., Omura S., Shiomi K.: Herquline A, produced by Penicillium herquei FKI-7215, exhibits anti-influenza virus properties. Biosci. Biotechnol. Biochem. 81 (1), 59-62 (2017).

平成２７（２０１５）年度

  1. Ito N., Nagai T., Hirose E., Kiyohara H., Oikawa T., Yamada H., Hanawa T.: Antidepressive-like effect of volatile components of kososan in a mouse model of stress-induced depression. Trad. Kampo Med. 3 (2), 87-93 (2016).
  2. Sekiya M., Kiyohara H., Maruyama H., Tsukada N., Yasuda A., Nagai T., Yamada H.: Anti-inflammatory effect of hospital-grade hochuekkito extract, a traditional Japanese herbal medicine, on methotrexate-induced intestinal mucositis in mice. Trad. Kampo Med. 2 (2), 81-88 (2015).
  3. Nagai T., Hashimoto R., Okuda S. M., Kodera Y., Oh-Ishi M., Maeda T., Ito N., Hanawa T., Kiyohara H., Yamada H.: Antidepressive-like effect of a Kampo (traditional Japanese) medicine, kososan (Xiang Su San) in a stress-induced depression-like mouse model: Proteomic analysis of hypothalamus. Trad. Kampo Med. 2 (2), 50-59 (2015).

平成２６（２０１４）年度

  1. Nagai T., Kataoka E., Aoki Y., Hokari R., Kiyohara H., Yamada H.: Alleviative effects of a Kampo (a Japanese herbal) medicine "maoto (Ma-Huang-Tang)" on the early phase of influenza virus infection and its possible mode of action. Evid. Based Complement. Alternat. Med. 2014, Article ID 187036, 12 pages (2014).
  2. Aoyagi Y., Fujiwara K., Yamazaki A., Sugawara N., Yano R., Fukaya H., Hitotsuyanagi Y., Takeya K., Ishiyama A., Iwatsuki M., Otoguro K., Yamada H., Omura S.: Semisynthesis of salviandulin E analogues and their antitrypanosomal activity. Bioorg. Med. Chem. Lett. 24 (2), 442-446 (2014).

平成２５（２０１３）年度

  1. Makino S., Ikegami S., Nagai T., Yamada H.: Immunogenics: Extracellular polysaccharides reduce the risk of infection. Probiotics: Immunobiotics and Immunogenics (Eds. Kitazawa H., Villena J., Alvarez S.), p. 376-397, CRC Press, Boca Raton, FL, USA (2013).
  2. Slavov A., Kiyohara H., Yamada H.: Immunomodulating pectic polysaccharides from waste rose petals of Rosa damascena Mill. Int. J. Biol. Macromol. 59, 192-200 (2013).
  3. Sekiya M., Kiyohara H., Maruyama H., Yabe T., Yamada H.: Modulation of chemokine expression on intestinal epithelial cells by Kampo (traditional Japanese herbal) medicine, Hochuekkito, and its active ingredients. J. Nat. Med. 67 (3), 626-635 (2013).

平成２４（２０１２）年度

  1. Hokari R., Nagai T., Yamada H.: In vivo anti-influenza virus activity of Japanese herbal (Kampo) medicine, "Shahakusan," and its possible mode of action. Evid. Based Complement. Alternat. Med. 2012, Article ID 794970, 13 pages (2012).
  2. Yamamoto T., Izumi N., Ui H., Sueki A., Masuma R., Nonaka K., Hirose T., Sunazuka T., Nagai T., Yamada H., Omura S., Shiomi K.: Wickerols A and B: novel anti-influenza virus diterpenes produced by Trichoderma atroviride FKI-3849. Tetrahedron 68 (45), 9267-9271 (2012).
  3. Ito N., Hori A., Yabe T., Nagai T., Oikawa T., Yamada H., Hanawa T.: Involvement of neuropeptide Y signaling in the antidepressant-like effect and hippocampal cell proliferation induced by kososan, a Kampo medicine, in the stress-induced depression-like model mice. Biol. Pharm. Bull. 35 (10), 1775-1783 (2012).
  4. Otoguro K., Ishiyama A., Iwatsuki M., Namatame M., Niashihara-Tsukashima A., Kiyohara H., Hashimoto T., Asakawa Y., Omura S., Yamada H.: In vitro antitrypanosomal activity of bis(bibenzyls)s and bibenzyls from liverworts against Trypanosoma brucei. J. Nat. Med. 66 (2), 377-382 (2012).

平成２３（２０１１）年度

  1. Ishiyama A., Otoguro K., Iwatsuki M., Namatame M., Nishihara-Tsukashima A., Takahashi Y., Onodera H., Yamada H., Omura S.: In vitro antitrypanosomal activity of five low-MW antibiotics. J. Antibiot. 65 (2), 113-114 (2012).
  2. Otoguro K., Iwatsuki M., Ishiyama A., Namatame M., Nishihara-Tsukashima A., Kiyohara H., Hashimoto T., Asakawa Y., Omura S., Yamada H.: In vitro antitrypanosomal activity of some phenolic compounds from propolis and lactones from Fijian Kawa (Piper methysticum). J. Nat. Med. 66 (3), 558-561 (2012).
  3. Kiyohara H., Ichino C., Kawamura Y., Nagai T., Sato N., Yamada H., Salama M.M., Abdel-Satta E.: In vitro anti-influenza virus activity of a cardiotonic glycoside from Adenium obesum (Forssk.). Phytomedicine 19 (2), 111-114 (2012).
  4. Kiyohara H., Ichino C., Kawamura Y., Nagai T., Sato N., Yamada H.: Patchouli alcohol: in vitro direct anti-influenza virus sesquiterpene in Pogostemon cablin Benth. J. Nat. Med. 66 (1), 55-61 (2012).
  5. Gronhaug T.E., Kiyohara H., Sveaass A., Diallo D., Yamada H., Paulsen B.S.: Beta-D-(1→4)-galactan-containing side chains in RG-I regions of pectic polysaccharides from Biophytum petersianum Klotzsch. contribute to expression of immunomodulating activity against intestinal Peyer’s patch cells and macrophages. Phytochemistry 72 (17), 2139–2147 (2011).
  6. Nagai T., Makino S., Ikegami S., Itoh H., Yamada H.: Effect of oral administration of yogurt fermented with Lactobacillus delbrueckiis sp. bulgaricus OLL1073R-1 and its polysaccharides against influenza virus infection in mice. Int. Immunopharmacol. 11 (12), 2246-2250 (2011).
  7. Otoguro K., Iwatsuki M., Ishiyama A., Namatame M., Nishihara-Tsukashima A., Kiyohara H., Hashimoto T., Asakawa Y., Omura S., Yamada H.: In vitro antitrypanosomal activity of plant terpenes against Trypanosoma brucei. Phytochemistry 72 (16), 2024-2030 (2011).
  8. Singharachai C., Palanuvej C., Kiyohara H., Yamada H., Ruangrungsi N.: Safety evaluation of Thai traditional medicine remedy: Ben-Chao-Lo-Ka-Wi-Chian. J. Health Res. 25, 83-90 (2011).
  9. Oguri H., Hiura T., Yamagishi Y., Oikawa H., Ishiyama A., Otoguro K., Yamada H., Omura S.: Generation of anti-trypanosomal agents through concise synthesis and structural diversification of sesquiterpene analogues. J. Am. Chem. Soc. 133 (18), 7096-7105 (2011).
  10. Inahashi Y., Iwatsuki M., Ishiyama A., Namatame M., Nishihara-Tsumashima A., Matsumoto A., Hirose T., Sunazuka T., Yamada H., Otoguro K., Takahashi Y., Omura S., Shiomi K.: Spoxazomicins A-C, novel antitrypanosomal alkaloids produced by an endophytic actinomycete, Streptosporangium oxazolium K07-040(T). J. Antibiot. 64 (4), 303-307 (2011).
  11. Singharachai C., Palanuvej C., Kiyohara H., Yamada H., Ruangrungsi N.: Pharmacognostic specification of five root species in Thai traditional medicine remedy: Ben-Cha-Lo-Ka-Wi-Chian. Pharmacog. J. 3, 1-11 (2011).

平成２２（２０１０）年度

  1. Namba T, Yabe T, Gonda Y, Ichikawa N, Sanagi T, Arikawa-Hirasawa E, Mochizuki H, Kohsaka S, Uchino S. : Pigment epithelium-derived factor up-regulation induced by memantine, an N-methyl-D-aspartate receptor antagonist, is involved in increased proliferation of hippocampal progenitor cells　Neuroscience　167 (2)：372-383　2010
  2. Otoguro K, Iwatsuki M, Ishiyama A, Namatame M, Nishihara-Tsukashima A, Sato S, Hatsu M, Iinuma H, Shibahara S, Nimura S, Kondo S, Yamada H, Omura S :In vitro and in vivo antiprotozoal activities of bispolides and their derivatives　J Antibiot (Tokyo)　63 (5)：275-277　2010
  3. Nagai T, Shimizu Y, Shirahata T, Sunazuka T, Kiyohara H, Omura S, Yamada H : Oral adjuvant activity for nasal influenza vaccines caused by combination of two trihydroxy fatty acid stereoisomers from the tuber of Pinellia ternata　Int Immunopharmacol　10 (6)：655-661　2010
  4. Iwatsuki M, Kinoshita Y, Niitsuma M, Hashida J, Mori M, Ishiyama A, Namatame M, Nishihara-Tsukashima A, Nonaka K, Masuma R, Otoguro K, Yamada H, Shiomi K, Omura S : Antitrypanosomal peptaibiotics, trichosporins B-VIIa and B-VIIb, produced by Trichoderma polysporum FKI-4452　J Antibiot (Tokyo)　63 (6)：331-333　2010
  5. Otoguro K, Iwatsuki M, Ishiyama A, Namatame M, Nishihara-Tukashima A, Shibahara S, Kondo S, Yamada H, Omura S : Promising lead compounds for novel antiprotozoals.　J Antibiot (Tokyo)　63 (7)：381-384　2010
  6. Matsumoto T, Moriya M, Kiyohara H, Tabuchi Y, Yamada H : Hochuekkito, a Kampo (traditional Japanese herbal) medicine, and its polysaccharide portion stimulate G-CSF secretion from intestinal epithelial cells　Evid Based Complement Alternat Med　7 (3)：331-340　2010
  7. Hashida J, Niitsuma M, Iwatsuki M, Mori M, Ishiyama A, Namatame M, Nishihara-Tsukashima A, Nonaka K, Ui H, Masuma R, Otoguro K, Yamada H, Shiomi K, Omura S : Pyrenocine I, a new pyrenocine analog produced by Paecilomyces sp. FKI-3573　J Antibiot (Tokyo)　63 (9)：559-561　2010
  8. Otoguro K, Ishiyama A, Iwatsuki M, Namatame M, Nishihara-Tukashima A, Nakashima T, Shibahara S, Kondo S, Yamada H, Omura S :In vitro and in vivo anti-Trypanosoma brucei activities of phenazinomycin and related compounds　J Antibiot (Tokyo)　63 (9)：579-581　2010
  9. Iwatsuki M, Otoguro K, Ishiyama A, Namatame M, Nishihara-Tukashima A, Hashida J, Nakashima T, Masuma R, Takahashi Y, Yamada H, Omura S :In vitro antitrypanosomal activity of 12 low-molecular-weight antibiotics and observations of structure/activity relationships　J Antibiot (Tokyo)　63 (10)：619-622　2010
  10. Niitsuma M, Hashida J, Iwatsuki M, Mori M, Ishiyama A, Namatame M, Nishihara-Tsukashima A, Matsumoto A, Takahashi Y, Yamada H, Otoguro K, Shiomi K, Omura S : Sinefungin VA and dehydrosinefungin V, new antitrypanosomal antibiotics produced by Streptomyces sp. K05-0178　J Antibiot (Tokyo)　63 (11)：673-679　2010
  11. Kiyohara H, Nonaka K, Sekiya M, Matsumoto T, Nagai T, Tabuchi Y, Yamada H : Polysaccharide-containing macromolecules in a Kampo (traditional Japanese herbal) medicine, Hochuekkito: Dual active ingredients for modulation of immune functions on intestinal Peyer's patches and epithelial cells　Evid Based Complement Alternat Med　2011：Article ID 492691, 13 pages　2011
  12. Nagai T, Nakao M, Shimizu Y, Kodera Y, Oh-Ishi M, Maeda T, Yamada H : Proteomic analysis of anti-inflammatory effects of a Kampo (Japanese herbal) medicine "Shoseiryuto (Xiao-Qing-Long-Tang)" on airway inflammation in a mouse model　Evid Based Complement Alternat Med　2011：Article ID 604196, 13 pages　2011
  13. Yabe T, Sanagi T, Yamada H : The neuroprotective role of PEDF: implication for the therapy of neurological disorders　Curr Mol Med　10 (3)：259-266　2010